The DIG RNA Labeling Kit SP6/T7 From Roche. The DIG RNA Labeling Kit is based on the in vitro transcription of a template cDNA. cDNA is cloned read more. Biocompare is the leading resource for up-to-date product information, product reviews, and . · Following sequence verification, antisense riboprobes were generated by in vitro transcription with a DIG RNA labeling kit Sp6/T7 (Roche). Antisense riboprobes were also generated following sequence verification through in vitro transcription with a DIG RNA labeling kit Sp6/T7 (Roche). The dre-let-7a probe was purchased from EXIQON. Price from $ to $ dig rna labeling kit sp6 t7 - by Bioz Stars, 86 / stars. Buy from Supplier. dig rna labeling kit (Roche) Roche dig rna labeling kit. Dig Rna Labeling Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/, based on 1 PubMed citations.
Both digoxin (Dig)-labeled HassOR31 probe and biotin (Bio)-labeled HassORco probe were synthesized with DIG RNA labeling kit version 12 (SP6/T7) (Roche, Mannheim, Germany), with Dig-NTP or Bio-NTP (Roche, Mannheim, Germany) labeling mixture, respectively. Anti-sense mGlu2 and mGlu3 in situ probes were generated by in vitro transcription with DIG-labeled NTPs from linearized cDNA templates, using DIG RNA Labeling SP6/T7 Kit (Roche, ) Similar Products. The reverse primer included a T7 promoter sequence to permit antisense RNA generation using the Roche DIG RNA Labeling kit (SP6/T7) (Roche Applied Science) according to manufacturer's instructions To prepare human tissue arrays, paraffin blocks containing formalin-fixed tissues from normal anonymous adult donors were purchased from.
DIG RNA Labeling Kit (SP6/T7) From Roche Applied Science. During my work, I had to make RNA probes for in situ hybridization in Xenopus embryos and I used this kit. This kit allows the DNA template to be transcribed and cloned into the polylinker site of appropriate transcription vectors, which contain promoters for SP6 and/or T7 RNA polymerases. The DIG RNA Labeling Kit is based on the in vitro transcription of a template cDNA. cDNA is cloned into the polylinker site of a transcription vector (supplied) containing SP6 and T7 promoters. Digoxigenin (DIG) -labeled UTP is incorporated (approximately in a ratio with UTP) into the transcript, generating DIG-labeled RNAs. 10 DIG RNA Labeling Kit (SP6/T7), RNase Inhibitor • Solution, 20 U/μl • Prevents the degradation of RNA during the labeling reaction. 1 vial, 20 μl 11 DIG RNA Labeling Kit (SP6/T7), SP6 RNA Polymerase • Solution, 20 U/μl • Synthesizes RNA from a DNA template. 1 vial, 20 μl 12 DIG RNA Labeling Kit (SP6/T7), T7 RNA Polymerase.