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Both digoxin (Dig)-labeled HassOR31 probe and biotin (Bio)-labeled HassORco probe were synthesized with DIG RNA labeling kit version 12 (SP6/T7) (Roche, Mannheim, Germany), with Dig-NTP or Bio-NTP (Roche, Mannheim, Germany) labeling mixture, respectively. Anti-sense mGlu2 and mGlu3 in situ probes were generated by in vitro transcription with DIG-labeled NTPs from linearized cDNA templates, using DIG RNA Labeling SP6/T7 Kit (Roche, ) Similar Products. The reverse primer included a T7 promoter sequence to permit antisense RNA generation using the Roche DIG RNA Labeling kit (SP6/T7) (Roche Applied Science) according to manufacturer's instructions To prepare human tissue arrays, paraffin blocks containing formalin-fixed tissues from normal anonymous adult donors were purchased from.


DIG RNA Labeling Kit (SP6/T7) From Roche Applied Science. During my work, I had to make RNA probes for in situ hybridization in Xenopus embryos and I used this kit. This kit allows the DNA template to be transcribed and cloned into the polylinker site of appropriate transcription vectors, which contain promoters for SP6 and/or T7 RNA polymerases. The DIG RNA Labeling Kit is based on the in vitro transcription of a template cDNA. cDNA is cloned into the polylinker site of a transcription vector (supplied) containing SP6 and T7 promoters. Digoxigenin (DIG) -labeled UTP is incorporated (approximately in a ratio with UTP) into the transcript, generating DIG-labeled RNAs. 10 DIG RNA Labeling Kit (SP6/T7), RNase Inhibitor • Solution, 20 U/μl • Prevents the degradation of RNA during the labeling reaction. 1 vial, 20 μl 11 DIG RNA Labeling Kit (SP6/T7), SP6 RNA Polymerase • Solution, 20 U/μl • Synthesizes RNA from a DNA template. 1 vial, 20 μl 12 DIG RNA Labeling Kit (SP6/T7), T7 RNA Polymerase.






















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The Differential Ability Scales – Second Edition (DAS-II; Elliott, ) is an individually administered, norm-referenced instrument that assesses the cognitive abilities of children and adolescents ages 2 years, 6 months through 17 years, 11 months. The DAS-II is useful for identifying strengths and weaknesses so appropriate individual. Differential Ability Scales. Differential Ability Scales. Sattler () Review of the DAS. 1. Good validity. 2. High reliabilities. 3. Excellent standardization. 4. Good administration procedures. 5. Good administrative guidelines and test materials. 6. Helpful scoring criteria. Sattler () Review of the DAS 1. Good validity 2. High. Overview of the Differential Ability Scales-Second Edition Administration and Scoring Manual 35 DAS-II Translations Administration directions for all.






















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the first step in development. Policy and procedure management is the process of developing, communicating, and organizing them so everyone has access and utilizes them in an agency. Having well managed and comprehensive policies and procedures supports the agencies programs, activities, a culture of compliance in. Created Date: 1/15/ PM.  · 9 Steps for Developing Policy/Procedure Manuals Use the following steps to jump-start the policy/procedure manual development process: 1. Obtain and review format and style standards for your organization's manuals. 2. Gather and analyze information: Review existing manuals to gauge the effects of the new/revised policies/procedures.


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